anti-CD15 antibody [MEM-158] (APC)

anti-CD15 antibody [MEM-158] (APC) for Flow cytometry and Human

Controls and Markers antibody


Product Description

APC-conjugated Mouse Monoclonal antibody [MEM-158] recognizes CD15

Tested Reactivity Hu
Species Does Not React With Bov, Pig, Sheep
Tested Application FACS
Specificity The clone MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
HLDA VI; WS Code AS A053
Host Mouse
Clonality Monoclonal
Clone MEM-158
Isotype IgM
Target Name CD15
Antigen Species Human
Immunogen Human granulocytes
Conjugation APC
Alternate Names LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer TBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 2526 Human FUT4

Swiss-port # P22083 Human Alpha-(1,3)-fucosyltransferase 4

Gene Symbol FUT4
Gene Full Name fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Background CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Function May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt]
Research Area Controls and Markers antibody
Calculated MW 59 kDa

Images (2) Click the Picture to Zoom In

  • ARG54270 anti-CD15 antibody [MEM-158] (APC) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG54270 anti-CD15 antibody [MEM-158] (APC) (10 µl reagent / 100 µl of peripheral whole blood).

  • ARG54270 anti-CD15 antibody [MEM-158] (APC) FACS image

    Flow Cytometry: Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG54270 anti-CD15 antibody [MEM-158] (APC) (10 µl reagent / 100 µl of peripheral whole blood).