ARG21001

anti-CD152 / CTLA4 antibody [1B8]

anti-CD152 / CTLA4 antibody [1B8] for ELISA,Flow cytometry and Mouse

Overview

Product Description Hamster Monoclonal antibody [1B8] recognizes CD152 / CTLA4
Tested Reactivity Ms
Tested Application ELISA, FACS
Specificity Mouse CD152.
Host Hamster
Clonality Monoclonal
Clone 1B8
Isotype IgG1
Target Name CD152 / CTLA4
Antigen Species Mouse
Immunogen Extracellular portion of murine CTLA-4 fused to a murine IgG2a
Conjugation Un-conjugated
Full Name cytotoxic T-lymphocyte-associated protein 4
Alternate Names GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACSAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 25 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12477 Mouse CTLA4

Swiss-port # P09793 Mouse Cytotoxic T-lymphocyte protein 4

Gene Symbol CTLA4
Gene Full Name cytotoxic T-lymphocyte-associated protein 4
Background This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
PTM N-glycosylation is important for dimerization.
Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.