ARG21004

anti-CD152 / CTLA4 antibody [1B8] (PE)

anti-CD152 / CTLA4 antibody [1B8] (PE) for Flow cytometry,FLISA and Mouse

Overview

Product Description PE-conjugated Hamster Monoclonal antibody [1B8] recognizes CD152 / CTLA4
Tested Reactivity Ms
Tested Application FACS, FLISA
Specificity Mouse CD152.
Host Hamster
Clonality Monoclonal
Clone 1B8
Isotype IgG1
Target Name CD152 / CTLA4
Antigen Species Mouse
Immunogen Extracellular portion of murine CTLA-4 fused to a murine IgG2a
Conjugation PE
Full Name cytotoxic T-lymphocyte-associated protein 4
Alternate Names GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 0.3 µg/10^6 cells
FLISA
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 25 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12477 Mouse CTLA4

Swiss-port # P09793 Mouse Cytotoxic T-lymphocyte protein 4

Gene Symbol CTLA4
Gene Full Name cytotoxic T-lymphocyte-associated protein 4
Background This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
PTM N-glycosylation is important for dimerization.
Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.

Images (1) Click the Picture to Zoom In

  • ARG21004 anti-CD152 / CTLA4 antibody [1B8] (PE) FLISA image

    FLISA: The plate was coated with Mouse CD152 / CTLA4 and purified/unlabeled Rat IgG1κ. CTLA4 and purified immunoglobulin were detected with serially diluted ARG21004 anti-CD152 / CTLA4 antibody [1B8] (PE).