anti-CD152 / CTLA4 antibody [SQab22255]

anti-CD152 / CTLA4 antibody [SQab22255] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab22255] recognizes CD152 / CTLA4
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab22255
Isotype IgG
Target Name CD152 / CTLA4
Antigen Species Human
Immunogen Synthetic peptide within Human CTLA-4.
Conjugation Un-conjugated
Protein Full Name Cytotoxic T-lymphocyte protein 4
Alternate Names GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human lymphoma tissue


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1493 Human CTLA4

Swiss-port # P16410 Human Cytotoxic T-lymphocyte protein 4

Gene Symbol CTLA4
Gene Full Name cytotoxic T-lymphocyte-associated protein 4
Background This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
Cellular Localization Cell membrane, Single-pass type I membrane protein. [UniProt]
Calculated MW 25 kDa
PTM N-glycosylation is important for dimerization.

Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG66927 anti-CD152 / CTLA4 antibody [SQab22255] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human lymphoma tissue stained with ARG66927 anti-CD152 / CTLA4 antibody [SQab22255]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).