anti-CD152 / CTLA4 antibody [SQab22255]
anti-CD152 / CTLA4 antibody [SQab22255] for IHC-Formalin-fixed paraffin-embedded sections and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab22255] recognizes CD152 / CTLA4|
|Target Name||CD152 / CTLA4|
|Immunogen||Synthetic peptide within Human CTLA-4.|
|Protein Full Name||Cytotoxic T-lymphocyte protein 4|
|Alternate Names||GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12|
|Application Note||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||Human lymphoma tissue|
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05%BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||cytotoxic T-lymphocyte-associated protein 4|
|Background||This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]|
|Function||Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]|
|Cellular Localization||Cell membrane, Single-pass type I membrane protein. [UniProt]|
|Calculated MW||25 kDa|
|PTM||N-glycosylation is important for dimerization.
Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt]
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