anti-CD158f / KIR2DL5 antibody [UP-R1] (PE)

anti-CD158f / KIR2DL5 antibody [UP-R1] (PE) for Flow cytometry and Human

Immune System antibody


Product Description PE-conjugated Mouse Monoclonal antibody [UP-R1] recognizes CD158f
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody UPR1 recognizes CD158f (KIR2DL5), a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.
Host Mouse
Clonality Monoclonal
Clone UP-R1
Isotype IgG1
Target Name CD158f / KIR2DL5
Antigen Species Human
Immunogen Human CD158f-Ig fusion protein
Conjugation PE
Alternate Names CD antigen CD158f1; KIR2DL5.3; KIR2DL5.1; Killer cell immunoglobulin-like receptor 2DL5A; KIR2DL5; CD158F

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 57292 Human KIR2DL5A

Swiss-port # Q8N109 Human Killer cell immunoglobulin-like receptor 2DL5A

Gene Symbol KIR2DL5A
Gene Full Name killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A
Background CD158f, also known as KIR2DL5, is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population.
Function Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. [UniProt]
Research Area Immune System antibody
Calculated MW 41 kDa

Images (1) Click the Picture to Zoom In

  • ARG54305 anti-CD158f / KIR2DL5 antibody [UP-R1] (PE) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG54305 anti-CD158f / KIR2DL5 antibody [UP-R1] (PE).