ARG52704

anti-CD16a antibody [SP189]

anti-CD16a antibody [SP189] for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody

Overview

Product Description Rabbit Monoclonal antibody [SP189] recognizes CD16a
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP189
Isotype IgG
Target Name CD16a
Antigen Species Human
Immunogen Synthetic peptide derived from the C-terminus of human CD16a protein.
Conjugation Un-conjugated
Alternate Names FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
IHC-P1:100
WB1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Lung, Tonsil, K562 Cell Lysate

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2214 Human FCGR3A

Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A

Background CD16 is a cluster of differentiation found on the surface of natural killer or polymorphonuclear leukocytes (PMN) cells, neutrophils, monocytes and macrophages. There are two CD16 isoform CD16a and CD16b. CD16a is expressed in monocytes, macrophages and NK cells and CD16b is expressed in neutrophils.
Cellular Localization Membrane
Highlight Related products:
CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Calculated MW 29 kDa
PTM Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
The soluble form is produced by a proteolytic cleavage.

Images (12) Click the Picture to Zoom In

  • ARG52704 anti-CD16a antibody [SP189] WB image

    Western Blot: K562 Cell Lysate stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Lung stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Spleen stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Stomach Adenocarcinoma stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Liver stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Thymus stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Liver Hepatocellular Carcinoma stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Placenta stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human B Cell Lymphoma stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Cervical Squamous Cell Carcinoma stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human Colon Adenocarcinoma stained with CD16a antibody [SP189] (ARG52704)

  • ARG52704 anti-CD16a antibody [SP189] IHC-P image

    Immunohistochemistry: Human HK Lymphoma stained with CD16a antibody [SP189] (ARG52704)