ARG21132

anti-CD172a / SIRP alpha antibody [74-22-15] (Biotin)

anti-CD172a / SIRP alpha antibody [74-22-15] (Biotin) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,ELISA and Pig

Cell Biology and Cellular Response antibody; Neuroscience antibody; Cardiomyocyte Cell Surface Marker antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [74-22-15] recognizes CD172a / SIRP alpha
Tested Reactivity Pig
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P
Specificity Porcine Monocytes & Granulocytes. The clone 74-22-15 reacts with > 90% of porcine monocytes and granulocytes and < 5% of peripheral blood lymphocytes.
Host Mouse
Clonality Monoclonal
Clone 74-22-15
Isotype IgG1, kappa
Target Name CD172a / SIRP alpha
Species Pig
Immunogen Fresh dd miniature swine thymocytes
Conjugation Biotin
Full Name signal-regulatory protein alpha
Alternate Names CD172A; p84; SHPS1; SHPS-1; CD172 antigen-like family member A; Sirp-alpha-3; Sirp-alpha-1; BIT; MYD-1; MFR; Bit; PTPNS1; CD antigen CD172a; Inhibitory receptor SHPS-1; SIRP; MyD-1 antigen; Sirp-alpha-2; Tyrosine-protein phosphatase non-receptor type substrate 1; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; Macrophage fusion receptor; Brain Ig-like molecule with tyrosine-based activation motifs; P84; SHP substrate 1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 55 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol SIRPA
Gene Full Name signal-regulatory protein alpha
Background The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]
Function Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein [UniProt]
Research Area Cell Biology and Cellular Response antibody; Neuroscience antibody; Cardiomyocyte Cell Surface Marker antibody
PTM N-glycosylated.

Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21132 anti-CD172a / SIRP alpha antibody [74-22-15] (Biotin) FACS image

    Flow Cytometry: Porcine small lymphocytes, monocytes, and granulocytes stained with ARG21132 anti-CD172a / SIRP alpha antibody [74-22-15] (Biotin) and ARG21114 anti-CD3e antibody [PPT3] (PE).