anti-CD177 antibody [MEM-166] (APC)

anti-CD177 antibody [MEM-166] (APC) for Flow cytometry and Human,Primates

Immune System antibody


Product Description

APC-conjugated Mouse Monoclonal antibody [MEM-166] recognizes CD177

Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
HLDA VI; WS Code M M17
HLDA VI; WS Code BP 309
Host Mouse
Clonality Monoclonal
Clone MEM-166
Isotype IgG1
Target Name CD177
Antigen Species Human
Immunogen Human granulocytes
Conjugation APC
Alternate Names NB1 glycoprotein; PRV-1; PRV1; NB1; CD177 antigen; Human neutrophil alloantigen 2a; NB1 GP; HNA2A; Polycythemia rubra vera protein 1; CD antigen CD177; HNA-2a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 57126 Human CD177

Swiss-port # Q8N6Q3 Human CD177 antigen

Gene Symbol CD177
Gene Full Name CD177 molecule
Background CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
Research Area Immune System antibody
Calculated MW 46 kDa
PTM N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

Images (1) Click the Picture to Zoom In

  • ARG53776 anti-CD177 antibody [MEM-166] (APC) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53776 anti-CD177 antibody [MEM-166] (APC).

Clone References

Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177.

WB, FACS / Human

Hu N et al.
PLoS One.,  (2014)




The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy.

IHC-P / Human

Stepensky P et al.
Blood.,  (2013)




Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Drukker M et al.
Nat Biotechnol.,  (2012)




Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration.

FACS / Human

Hu N et al.
Arthritis Res Ther.,  (2011)




CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses.

FACS / Human

Zhou J et al.
J Exp Med.,  (2010)