anti-CD178 / Fas Ligand antibody [NOK-1] (PE)

anti-CD178 / Fas Ligand antibody [NOK-1] (PE) for Flow cytometry and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Immune System antibody


Product Description PE-conjugated Mouse Monoclonal antibody [NOK-1] recognizes CD178 / Fas Ligand
Tested Reactivity Hu
Tested Application FACS
Specificity The clone NOK-1 recognizes CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.

HCDM Workshop: VII 70322
Host Mouse
Clonality Monoclonal
Clone NOK-1
Isotype IgG1
Target Name CD178 / Fas Ligand
Antigen Species Human
Immunogen L5178Y mouse T lymphoma cells expressing recombinant human CD178
Conjugation PE
Alternate Names FasL ICD; SPPL2A-processed FasL form; Apoptosis antigen ligand; CD95 ligand; CD178; Fas antigen ligand; CD95-L; Receptor-binding FasL ectodomain; FasL; SPA; TNFSF6; CD95L; FASL; Fas ligand; APTL; APT1LG1; ALPS1B; sFasL; Soluble Fas ligand; Tumor necrosis factor ligand superfamily member 6; APL; CD antigen CD178

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 356 Human FASLG

Swiss-port # P48023 Human Tumor necrosis factor ligand superfamily member 6

Gene Symbol FASLG
Gene Full Name Fas ligand (TNF superfamily, member 6)
Background CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo.
Function Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 31 kDa
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260).
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.

Images (1) Click the Picture to Zoom In

  • ARG54289 anti-CD178 / Fas Ligand antibody [NOK-1] (PE) FACS image

    Flow Cytometry: Separation of CD178 transfected L5178Y cells (red) from non-transfected L5178Y cells (black-dashed). Cells were stained with ARG54289 anti-CD178 / Fas Ligand antibody [NOK-1] (PE) at 1 µg/ml dilution.