anti-CD178 / Fas Ligand antibody [SB93a] (FITC)
anti-CD178 / Fas Ligand antibody [SB93a] (FITC) for Flow cytometry,IHC-Frozen sections,ICC/IF,Western blot and Human
|Product Description||FITC-conjugated Mouse Monoclonal antibody [SB93a] recognizes CD178 / Fas Ligand|
|Tested Application||FACS, ICC/IF, IHC-Fr, WB|
|Specificity||Human/Chicken Fas ligand. The clone SB93a detects a band at ~40 kDa corresponding to the transmembrane/insoluble form of Fas ligand.|
|Target Name||CD178 / Fas Ligand|
|Immunogen||Recombinant human Fas ligand|
|Alternate Names||FasL ICD; SPPL2A-processed FasL form; Apoptosis antigen ligand; CD95 ligand; CD178; Fas antigen ligand; CD95-L; Receptor-binding FasL ectodomain; FasL; SPA; TNFSF6; CD95L; FASL; Fas ligand; APTL; APT1LG1; ALPS1B; sFasL; Soluble Fas ligand; Tumor necrosis factor ligand superfamily member 6; APL; CD antigen CD178|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Buffer||PBS and 0.1% Sodium azide.|
|Preservative||0.1% Sodium azide|
|Storage Instruction||Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||Fas ligand (TNF superfamily, member 6)|
|Background||This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]|
|Function||Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition. [UniProt]
|Calculated MW||31 kDa|
|PTM||The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260).
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
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