anti-CD18 / LFA1 beta antibody [MEM-148] (PE)

anti-CD18 / LFA1 beta antibody [MEM-148] (PE) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody


Product Description PE-conjugated Mouse Monoclonal antibody [MEM-148] recognizes CD18 / LFA1 beta
Tested Reactivity Hu
Species Does Not React With Pig
Tested Application FACS
Specificity The clone MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
HLDA VI; WS Code AS A052
Host Mouse
Clonality Monoclonal
Clone MEM-148
Isotype IgG1
Target Name CD18 / LFA1 beta
Immunogen Peripheral blood mononuclear cells
Conjugation PE
Alternate Names MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3689 Human ITGB2

Swiss-port # P05107 Human Integrin beta-2

Gene Symbol ITGB2
Gene Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 85 kDa
PTM Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).

Images (1) Click the Picture to Zoom In

  • ARG53781 anti-CD18 / LFA1 beta antibody [MEM-148] (PE) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG53781 anti-CD18 / LFA1 beta antibody [MEM-148] (PE).

Clone References

LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.

BL / Human

Ghislin S et al.
BMC Cancer.,  (2012)




The context of HLA-DR/CD18 complex in the plasma membrane governs HLA-DR-derived signals in activated monocytes.

Doisne JM et al.
Mol Immunol.,  (2008)




Indirubin, a Chinese anti-leukaemia drug, promotes neutrophilic differentiation of human myelocytic leukaemia HL-60 cells.

WB / Human

Suzuki K et al.
Br J Haematol.,  (2005)




A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells.

Drbal K et al.
Blood.,  (2001)




A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins.

Drbal K et al.
Immunobiology.,  (2001)




Human leukocytes contain a large pool of free forms of CD18.

Drbal K et al.
Biochem Biophys Res Commun.,  (2000)