anti-CD180 / RP105 antibody [G28-8] (APC)

anti-CD180 / RP105 antibody [G28-8] (APC) for Flow cytometry and Human

Immune System antibody; Microbiology and Infectious Disease antibody


Product Description APC-conjugated Mouse Monoclonal antibody [G28-8] recognizes CD180
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody G288 recognizes CD180, a 95105 kDa TLRlike glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
Host Mouse
Clonality Monoclonal
Clone G28-8
Isotype IgG1
Target Name CD180 / RP105
Antigen Species Human
Immunogen Human tonsillar B cells
Conjugation APC
Alternate Names LY64; Radioprotective 105 kDa protein; CD180 antigen; CD antigen CD180; Lymphocyte antigen 64; RP105; Ly78

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 4064 Human CD180

Swiss-port # Q99467 Human CD180 antigen

Gene Symbol CD180
Gene Full Name CD180 molecule
Background CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1 , and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.
Function May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity). [UniProt]
Research Area Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 74 kDa

Images (1) Click the Picture to Zoom In

  • ARG54246 anti-CD180 / RP105 antibody [G28-8] (APC) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG54246 anti-CD180 / RP105 antibody [G28-8] (APC) and anti-CD19 antibody (PE).

Clone References

Caspase activity is required for stimulated B lymphocytes to enter the cell cycle.

Olson NE et al.
J Immunol.,  (2003)




Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation.

Valentine MA et al.
J Immunol.,  (1988)