ARG46779
anti-CD19 antibody
anti-CD19 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes CD19 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Specificity | Using spleen and Jurkat cell lysates detects a 95 kDa band by Western blot. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD19 |
| Antigen Species | Human |
| Immunogen | Purified recombinant peptide within residues 510 aa to the C-terminus of human CD19 produced in E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD antigen CD19; CVID3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 95 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Epitope affinity purified |
| Buffer | PBS, 0.05% Sodium azide and 20% Glycerol. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20% Glycerol |
| Concentration | 3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD19 |
| Gene Full Name | CD19 antigen |
| Background | CD19: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008] |
| Function | CD19 functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701). [UniProt] |
| Calculated MW | 61 kDa |
| PTM | Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. |
Images (6) Click the Picture to Zoom In
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ARG46779 anti-CD19 antibody IHC-P image
Immunohistochemistry: Human appendix stained with ARG46779 anti-CD19 antibody at 1:750 dilution.
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ARG46779 anti-CD19 antibody IHC-P image
Immunohistochemistry: Human lymph node stained with ARG46779 anti-CD19 antibody at 1:750 dilution.
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ARG46779 anti-CD19 antibody IHC-P image
Immunohistochemistry: Mouse kidney stained with ARG46779 anti-CD19 antibody at 1:750 dilution.
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ARG46779 anti-CD19 antibody IHC-P image
Immunohistochemistry: Mouse liver stained with ARG46779 anti-CD19 antibody at 1:750 dilution.
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ARG46779 anti-CD19 antibody IHC-P image
Immunohistochemistry: Mouse spleen stained with ARG46779 anti-CD19 antibody at 1:750 dilution.
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ARG46779 anti-CD19 antibody WB image
Western blot: Mouse spleen stained with ARG46779 anti-CD19 antibody at 1:500 dilution.
