anti-CD203c / E-NPP3 antibody [NP4D6] (APC)

anti-CD203c / E-NPP3 antibody [NP4D6] (APC) for Flow cytometry and Human

Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody


Product Description

APC-conjugated Mouse Monoclonal antibody [NP4D6] recognizes CD203c

Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody NP4D6 reacts with CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.
Host Mouse
Clonality Monoclonal
Clone NP4D6
Isotype IgG1
Target Name CD203c / E-NPP3
Antigen Species Human
Immunogen HEK-293 cells transfected with human CD203c_x000D_
Conjugation APC
Alternate Names Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; PDNP3; NPPase; EC; EC; PD-Ibeta; PD-IBETA; NPP3; B10; CD antigen CD203c; Phosphodiesterase I beta; E-NPP 3; Phosphodiesterase I/nucleotide pyrophosphatase 3; CD203c

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5169 Human ENPP3

Swiss-port # O14638 Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Gene Symbol ENPP3
Gene Full Name ectonucleotide pyrophosphatase/phosphodiesterase 3
Background CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.
Function Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD. [UniProt]
Research Area Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 100 kDa
PTM N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.

Images (2) Click the Picture to Zoom In

  • ARG54290 anti-CD203c / E-NPP3 antibody [NP4D6] (APC) FACS image

    Flow Cytometry: Human basophils in IgE-activated whole blood stained with ARG54290 anti-CD203c / E-NPP3 antibody [NP4D6] (APC).

  • ARG54290 anti-CD203c / E-NPP3 antibody [NP4D6] (APC) FACS image

    Flow Cytometry: IgE-stimulated human PBMC stained with anti-CD107a antibody [H4A3] (Pacific Blue) and ARG54290 anti-CD203c / E-NPP3 antibody [NP4D6] (APC).

Clone References

Ecto-nucleotidases distribution in human cyclic and postmenopausic endometrium.

IHC-Fr / Human

Aliagas E et al.
Purinergic Signal.,  (2013)