ARG20867

anti-CD22 antibody [2D6]

anti-CD22 antibody [2D6] for Cellular activation ,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Mouse

Cancer antibody; Developmental Biology antibody; Immune System antibody; Immature B Cell Marker antibody

Overview

Product Description Rat Monoclonal antibody [2D6] recognizes CD22
Tested Reactivity Ms
Tested Application Cell-Act , FACS, ICC/IF, IHC-Fr, IHC-P, IP
Specificity Mouse CD22.
Host Rat
Clonality Monoclonal
Clone 2D6
Isotype IgG1, kappa
Target Name CD22
Antigen Species Mouse
Immunogen Splenic lymphocyte plasma membranes from CBA x C57 F1 mice
Conjugation Un-conjugated
Full Name CD22 antigen
Alternate Names B-lymphocyte cell adhesion molecule; B-cell receptor CD22; T-cell surface antigen Leu-14; BL-CAM; SIGLEC-2; Sialic acid-binding Ig-like lectin 2; Siglec-2; CD antigen CD22; SIGLEC2

Application Instructions

Application Suggestion
Tested Application Dilution
Cell-Act Assay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 95 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12483 Mouse CD22

Swiss-port # P35329 Mouse B-cell receptor CD22

Gene Symbol CD22
Gene Full Name CD22 antigen
Function Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [UniProt]
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Immature B Cell Marker antibody
PTM Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN.

Images (1) Click the Picture to Zoom In

  • ARG20867 anti-CD22 antibody [2D6] FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20867 anti-CD22 antibody [2D6] followed by ARG21697 Mouse anti-Rat IgG1 antibody [G17E7] (Biotin) and Streptavidin (FITC).