ARG20872

anti-CD22 antibody [2D6] (PE-Cyanine 5)

anti-CD22 antibody [2D6] (PE-Cyanine 5) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Cellular activation and Mouse

Cancer antibody; Developmental Biology antibody; Immune System antibody; Immature B Cell Marker antibody

Overview

Product Description PE-Cyanine 5-conjugated Rat Monoclonal antibody [2D6] recognizes CD22
Tested Reactivity Ms
Tested Application Cell-Act , FACS, ICC/IF, IHC-Fr, IHC-P
Specificity Mouse CD22.
Host Rat
Clonality Monoclonal
Clone 2D6
Isotype IgG1, kappa
Target Name CD22
Antigen Species Mouse
Immunogen Splenic lymphocyte plasma membranes from CBA x C57 F1 mice
Conjugation PE-Cyanine 5
Full Name CD22 antigen
Alternate Names B-lymphocyte cell adhesion molecule; B-cell receptor CD22; T-cell surface antigen Leu-14; BL-CAM; SIGLEC-2; Sialic acid-binding Ig-like lectin 2; Siglec-2; CD antigen CD22; SIGLEC2

Application Instructions

Application Suggestion
Tested Application Dilution
Cell-Act Assay-dependent
FACS< 0.2 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 95 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12483 Mouse CD22

Swiss-port # P35329 Mouse B-cell receptor CD22

Gene Symbol CD22
Gene Full Name CD22 antigen
Function Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [UniProt]
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Immature B Cell Marker antibody
PTM Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN.