anti-CD222 / IGF2R antibody [MEM-238] (PE)

anti-CD222 / IGF2R antibody [MEM-238] (PE) for Flow cytometry and Human,Primates

Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody


Product Description PE-conjugated Mouse Monoclonal antibody [MEM-238] recognizes CD222 / IGF2R
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
Host Mouse
Clonality Monoclonal
Clone MEM-238
Isotype IgG1
Target Name CD222 / IGF2R
Immunogen Recombinant Vaccinia virus encoding CD222.
Conjugation PE
Alternate Names CD222; MPR 300; Insulin-like growth factor II receptor; M6P/IGF2R; MPRI; 300 kDa mannose 6-phosphate receptor; IGF-II receptor; CI Man-6-P receptor; M6P/IGF2 receptor; MPR1; CIMPR; Cation-independent mannose-6-phosphate receptor; CD antigen CD222; Insulin-like growth factor 2 receptor; M6P-R; CI-MPR; M6PR

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3482 Human IGF2R

Swiss-port # P11717 Human Cation-independent mannose-6-phosphate receptor

Gene Symbol IGF2R
Gene Full Name insulin-like growth factor 2 receptor
Background CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
Function Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. [UniProt]
Research Area Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 274 kDa

Images (1) Click the Picture to Zoom In

  • ARG54276 anti-CD222 / IGF2R antibody [MEM-238] (PE) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG54276 anti-CD222 / IGF2R antibody [MEM-238] (PE) (blue) or Mouse IgG1 Isotype control antibody (PE) (red).

Clone References

Alix serves as an adaptor that allows human parainfluenza virus type 1 to interact with the host cell ESCRT system.

ICC/IF / Human

Boonyaratanakornkit J et al.
PLoS One.,  (2013)




The C proteins of human parainfluenza virus type 1 block IFN signaling by binding and retaining Stat1 in perinuclear aggregates at the late endosome.


Schomacker H et al.
PLoS One.,  (2012)




Histidine-rich glycoprotein binds heparanase and regulates its enzymatic activity and cell surface interactions.

FACS / Human

Poon IK et al.
Int J Biochem Cell Biol.,  (2010)




The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.

ICC/IF / Human

Hong Z et al.
Cell Res.,  (2009)




Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression.

Inhib / Human

Laube F et al.
Anticancer Res.,  (2009)




Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation.

IP / Mouse

Wood RJ et al.
J Biol Chem.,  (2008)