ARG20880

anti-CD25 antibody [7D4] (FITC)

anti-CD25 antibody [7D4] (FITC) for Flow cytometry,IHC-Frozen sections,Neutralizing,Blocking,Depletion and Mouse

Immune System antibody; Pre-B Cell Marker antibody

Overview

Product Description FITC-conjugated Rat Monoclonal antibody [7D4] recognizes CD25
Tested Reactivity Ms
Tested Application BL, Depletion, FACS, IHC-Fr, Neut
Specificity Mouse CD25. The clone 7D4 reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by The clone 3C7. While The clone 7D4 does not block the binding of IL-2 to CD25, when used in combination with The clone 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.
Host Rat
Clonality Monoclonal
Clone 7D4
Isotype IgM, kappa
Target Name CD25
Antigen Species Mouse
Immunogen IL-2 dependent BALB/c helper T cell clone HT2
Conjugation FITC
Full Name interleukin 2 receptor, alpha chain
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
DepletionAssay-dependent
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
NeutAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 31 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16184 Mouse IL2RA

Swiss-port # P01590 Mouse Interleukin-2 receptor subunit alpha

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha chain
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Research Area Immune System antibody; Pre-B Cell Marker antibody