anti-CD25 antibody [MEM-181] (PE)

anti-CD25 antibody [MEM-181] (PE) for Flow cytometry and Human

Immune System antibody; Pre-B Cell Marker antibody


Product Description PE-conjugated Mouse Monoclonal antibody [MEM-181] recognizes CD25
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
HLDA VI; WS Code NL N-L024
Host Mouse
Clonality Monoclonal
Clone MEM-181
Isotype IgG1
Target Name CD25
Immunogen PHA-activated peripheral blood leucocytes
Conjugation PE
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3559 Human IL2RA

Swiss-port # P01589 Human Interleukin-2 receptor subunit alpha

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha
Background CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
Function Receptor for interleukin-2. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30307 Pre-B Cell Marker Antibody Panel (CD19, CD25, CD38, CD40, CD45) (FACS)
Related products:
CD25 antibodies; CD25 ELISA Kits; CD25 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Immune System antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa

Images (1) Click the Picture to Zoom In

  • ARG53801 anti-CD25 antibody [MEM-181] (PE) FACS image

    Flow Cytometry: PHA-stimulated Human PBMC stained with ARG53801 anti-CD25 antibody [MEM-181] (PE).

Clone References

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

ICC/IF / Human

Brusés JL et al.
PLoS One.,  (2013)




Different motifs regulate trafficking of SorCS1 isoforms.

ICC/IF / Mouse

Nielsen MS et al.
Traffic.,  (2008)




Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.

Drbal K et al.
Int Immunol.,  (2007)