anti-CD253 / TRAIL antibody [2E5] (PE)

anti-CD253 / TRAIL antibody [2E5] (PE) for Flow cytometry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody


Product Description PE-conjugated Mouse Monoclonal antibody [2E5] recognizes CD253 / TRAIL
Tested Reactivity Hu
Species Does Not React With Ms
Tested Application FACS
Specificity The clone 2E5 reacts with TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.
Host Mouse
Clonality Monoclonal
Clone 2E5
Isotype IgG1
Target Name CD253 / TRAIL
Antigen Species Human
Immunogen Recombinant soluble fragment (aa 95-281) of human TRAIL.
Conjugation PE
Alternate Names TL2; CD253; Protein TRAIL; TNF-related apoptosis-inducing ligand; TRAIL; CD antigen CD253; Apo-2 ligand; Apo-2L; APO2L; Tumor necrosis factor ligand superfamily member 10

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 8743 Human TNFSF10

Swiss-port # P50591 Human Tumor necrosis factor ligand superfamily member 10

Gene Symbol TNFSF10
Gene Full Name tumor necrosis factor (ligand) superfamily, member 10
Background Human CD253 / TRAIL (TNF-Related Apoptosis Inducing Ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).
Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 33 kDa
PTM Tyrosine phosphorylated by PKDCC/VLK.

Clone References

Complex changes in the apoptotic and cell differentiation programs during initiation of the hair follicle response to chemotherapy.

Sharova TY et al.
J Invest Dermatol.,  (2014)




Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells.

Hyer ML et al.
Cancer Res.,  (2005)




TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors.

Plasilova M et al.
Leukemia.,  (2002)