ARG54196
anti-CD253 / TRAIL antibody [2E5] (PE)
anti-CD253 / TRAIL antibody [2E5] (PE) for Flow cytometry and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [2E5] recognizes CD253 / TRAIL |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms |
| Tested Application | FACS |
| Specificity | The clone 2E5 reacts with TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2E5 |
| Isotype | IgG1 |
| Target Name | CD253 / TRAIL |
| Antigen Species | Human |
| Immunogen | Recombinant soluble fragment (aa 95-281) of human TRAIL. |
| Conjugation | PE |
| Alternate Names | TL2; CD253; Protein TRAIL; TNF-related apoptosis-inducing ligand; TRAIL; CD antigen CD253; Apo-2 ligand; Apo-2L; APO2L; Tumor necrosis factor ligand superfamily member 10 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P50591 Human Tumor necrosis factor ligand superfamily member 10 |
|---|---|
| Gene Symbol | TNFSF10 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 10 |
| Background | Human CD253 / TRAIL (TNF-Related Apoptosis Inducing Ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2). |
| Function | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 33 kDa |
| PTM | Tyrosine phosphorylated by PKDCC/VLK. |
Clone References
Complex changes in the apoptotic and cell differentiation programs during initiation of the hair follicle response to chemotherapy.
Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells.
TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors.
