anti-CD27 antibody [LT27] (PE)

anti-CD27 antibody [LT27] (PE) for Flow cytometry and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody


Product Description PE-conjugated Mouse Monoclonal antibody [LT27] recognizes CD27
Tested Reactivity Hu
Tested Application FACS
Specificity The clone LT27 reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.
HLDA V; WS Code T T-CD27.01
Host Mouse
Clonality Monoclonal
Clone LT27
Isotype IgG2a
Target Name CD27
Antigen Species Human
Immunogen Human peripheral blood lymphocytes
Conjugation PE
Alternate Names T-cell activation antigen CD27; Tp55; CD antigen CD27; CD27 antigen; S152. LPFS2; T14; S152; Tumor necrosis factor receptor superfamily member 7; CD27L receptor; TNFRSF7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 939 Human CD27

Swiss-port # P26842 Human CD27 antigen

Gene Symbol CD27
Gene Full Name CD27 molecule
Background CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.
Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. [UniProt]
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 29 kDa
PTM Phosphorylated.
O-glycosylated with core 1 or possibly core 8 glycans.

Images (1) Click the Picture to Zoom In

  • ARG53813 anti-CD27 antibody [LT27] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53813 anti-CD27 antibody [LT27] (PE).

Clone References

Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion.

Sembries S et al.
Blood.,  (1999)