ARG54286

anti-CD271 / NGFR p75 antibody [NGFR5] (APC)

anti-CD271 / NGFR p75 antibody [NGFR5] (APC) for Flow cytometry and Cat,Ferret,Human,Primates,Rabbit

Developmental Biology antibody; Neuroscience antibody

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [NGFR5] recognizes CD271 / NGFR p75
Tested Reactivity Hu, Cat, Frt, NHuPrm, Rb
Species Does Not React With Ms, Rat
Tested Application FACS
Specificity The mouse monoclonal antibody NGFR5 (originally C34C) recognizes CD271 / NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily. The epitope is localized within amino acids 1 - 160.
Host Mouse
Clonality Monoclonal
Clone NGFR5
Isotype IgG1
Target Name CD271 / NGFR p75
Antigen Species Human
Immunogen Purified CD271 protein isolated from human melanoma cell line A875
Conjugation APC
Alternate Names Gp80-LNGFR; CD271; CD antigen CD271; p75 ICD; Tumor necrosis factor receptor superfamily member 16; p75NTR; NGF receptor; TNFRSF16; p75(NTR); Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4804 Human NGFR

Swiss-port # P08138 Human Tumor necrosis factor receptor superfamily member 16

Gene Symbol NGFR
Gene Full Name nerve growth factor receptor
Background CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.
Function Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]
Research Area Developmental Biology antibody; Neuroscience antibody
Calculated MW 45 kDa
PTM N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues.

Images (1) Click the Picture to Zoom In

  • ARG54286 anti-CD271 / NGFR p75 antibody [NGFR5] (APC) FACS image

    Flow Cytometry: REH cells stained with ARG54286 anti-CD271 / NGFR p75 antibody [NGFR5] (APC).

Clone References

Induced periosteum a complex cellular scaffold for the treatment of large bone defects.

IHC-P, FACS / Human

Cuthbert RJ et al.
Bone.,  (2013)

publication_link

 

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Pathology of experimental aerosol Zaire ebolavirus infection in rhesus macaques.

Twenhafel NA et al.
Vet Pathol.,  (2013)

publication_link

 

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The dimensions and characteristics of the subepidermal nerve plexus in human skin--terminal Schwann cells constitute a substantial cell population within the superficial dermis.

ICC/IF / Human

Reinisch CM et al.
J Dermatol Sci.,  (2012)

publication_link

 

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Rac1b increases with progressive tau pathology within cholinergic nucleus basalis neurons in Alzheimer's disease.

IHC / Human

Perez SE et al.
Am J Pathol.,  (2012)

publication_link

 

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Growth of benign and malignant schwannoma xenografts in severe combined immunodeficiency mice.

IHC / Mouse

Chang LS et al.
Laryngoscope.,  (2006)

publication_link

 

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Modulation of Fas-induced apoptosis by p75 neurotrophin receptor in a human neuroblastoma cell line.

WB / Human

Giraud S et al.
Apoptosis.,  (2005)

publication_link

 

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Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells.

FACS / Human

Yagi H et al.
Int Immunol.,  (2004)

publication_link

 

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