ARG65862

anti-CD274 / PD-L1 antibody [SQab1716]

anti-CD274 / PD-L1 antibody [SQab1716] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1716] recognizes CD274 / PD-L1
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP
Host Rabbit
Clonality Monoclonal
Clone SQab1716
Isotype IgG
Target Name CD274 / PD-L1
Species Human
Immunogen Recombinant protein around aa. 19-239 (extracellular) of Human CD274 / PD-L1.
Conjugation Un-conjugated
Full Name CD274 molecule
Alternate Names Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:200
ICC/IF1:1000 - 1:10000
IHC-P1:100 - 1:200
IP1:30
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 33 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 29126 Human CD274

Swiss-port # Q9NZQ7 Human Programmed cell death 1 ligand 1

Gene Symbol CD274
Gene Full Name CD274 molecule
Background CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function CD274 / PD-L1 plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [UniProt]
Cellular Localization Cell membrane and Endomembrane system.
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Images (6) Click the Picture to Zoom In

  • ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] ICC/IF image

    Immunofluorescence: 293 cells trasnfected with PD-L1 gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at room temperature. Samples were stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] (green) at 1:10000 at 4°C. DAPI (blue) was used as the nuclear counter stain.

    Control: PBS and secondary antibody.

  • ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] FACS image

    Flow Cytometry: 293 cells transfected with PD-L1 gene and stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:200, in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody (red histogram). Unlabelled sample (black histogram) was used as a control.

  • ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human placenta tissue stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] IP image

    Immunoprecipitation: PD-L1 was immunoprecipitated from 0.2 mg of PD-L1 gene transfected 293 whole cells lysate by ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:30 dilution. The immunoprecipitated lysates were then stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] by WB.

    Lane 1: ARG65862 IP in 293 whole cell lysate transfected with PD-L1 gene.
    Lane 2: PBS instead of ARG65862 in 293 whole cell lysate transfected with PD-L1 gene.
    Lane 3: 293 whole cell lysate transfected with PD-L1 gene, 2 μg (input).

  • ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human hepatocellular carcinoma tissue stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human lung adenocarcinoma tissue stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).