anti-CD279 / PD-1 antibody [SQab1732]

anti-CD279 / PD-1 antibody [SQab1732] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab1732] recognizes CD279 / PD-1
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP
Host Rabbit
Clonality Monoclonal
Clone SQab1732
Isotype IgG
Target Name CD279 / PD-1
Antigen Species Human
Immunogen Recombinant full length protein of Human PD‐1. The immunogen contains the specific extracellular domain of Human PD‐1 (Leu25 ‐ Gln167).
Conjugation Un-conjugated
Alternate Names hPD-l; CD279; PD-1; Protein PD-1; CD antigen CD279; PD1; hSLE1; SLEB2; Programmed cell death protein 1; hPD-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:200
ICC/IF1:2000 - 1:10000
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5133 Human PDCD1

Swiss-port # Q15116 Human Programmed cell death protein 1

Gene Symbol PDCD1
Gene Full Name programmed cell death 1
Background CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
Function CD279 / PD-1 is an inhibitory receptor on antigen activated T-cells. It plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552). [UniProt]
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Calculated MW 32 kDa

Images (4) Click the Picture to Zoom In

  • ARG66243 anti-CD279 / PD-1 antibody [SQab1732] ICC/IF image

    Immunofluorescence: 293 cells transfected with PD-1 gene, fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66243 anti-CD279 / PD-1 antibody [SQab1732] (green) at 1:10000 and 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG66243 anti-CD279 / PD-1 antibody [SQab1732] FACS image

    Flow Cytometry: 293 cells transfected with PD-1 gene. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66243 anti-CD279 / PD-1 antibody [SQab1732] (blue) at 1:200 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG66243 anti-CD279 / PD-1 antibody [SQab1732] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung tonsil tissue stained with ARG66243 anti-CD279 / PD-1 antibody [SQab1732] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG66243 anti-CD279 / PD-1 antibody [SQab1732] IP image

    Immunoprecipitation: 0.2 mg of 293 whole cell lysate (transfected with PD-1 gene) was immunoprecipitated (1:50 dilution) by ARG66243 anti-CD279 / PD-1 antibody [SQab1732] and then stained with anti-CD279 / PD-1 antibody.

    Lane 1: Immunoprecipitation in 293 whole cell lysate (transfected with PD-1 gene)
    Lane 2: PBS instead of Primary Ab in 293 whole cell lysate (transfected with PD-1 gene)
    Lane 3: 293 whole cell lysate (transfected with PD-1 gene), 2 µg (input)