anti-CD28 antibody [CD28.2] (PerCP)

anti-CD28 antibody [CD28.2] (PerCP) for Flow cytometry and Human,Primates

Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody


Product Description

PerCP-conjugated Mouse Monoclonal antibody [CD28.2] recognizes CD28

Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
HLDA V.; WS Code 5T CD28.05
Host Mouse
Clonality Monoclonal
Clone CD28.2
Isotype IgG1
Target Name CD28
Antigen Species Human
Immunogen DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Conjugation PerCP
Alternate Names Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 940 Human CD28

Swiss-port # P10747 Human T-cell-specific surface glycoprotein CD28

Gene Symbol CD28
Gene Full Name CD28 molecule
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Calculated MW 25 kDa
PTM CD40LG induces tyrosine phosphorylation of isoform 3.

Images (1) Click the Picture to Zoom In

  • ARG53815 anti-CD28 antibody [CD28.2] (PerCP) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53815 anti-CD28 antibody [CD28.2] (PerCP).

Clone References

Antipsychotic drugs suppress the AKT/NF-κB pathway and regulate the differentiation of T-cell subsets.

FuncSt / Human

Chen ML et al.
Immunol Lett.,  (2011)




NFATc3 regulates the transcription of genes involved in T-cell activation and angiogenesis.

Urso K et al.
Blood.,  (2011)




Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates.

Jeong SH et al.
J Virol.,  (2004)




Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling.

Scharschmidt E et al.
Mol Cell Biol.,  (2004)




Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6.

Marti F et al.
J Immunol.,  (2001)