ARG20469

anti-CD284 / TLR4 antibody

anti-CD284 / TLR4 antibody for Western blot and Rat

Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CD284 / TLR4
Tested Reactivity Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD284 / TLR4
Antigen Species Rat
Immunogen Synthetic peptide around 819 aa of Rat TLR4 protein (NP_062051.1)
Conjugation Un-conjugated
Full Name toll-like receptor 4
Alternate Names CD284; CD antigen CD284; ARMD10; hToll; TLR-4; TOLL; Toll-like receptor 4

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 96 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 29260 Rat TLR4

Swiss-port # Q9QX05 Rat Toll-like receptor 4

Gene Symbol Tlr4
Gene Full Name toll-like receptor 4
Background TLR4 (Toll-like receptor 4) is a mammalain homologue of the Drosophila toll protein. The human TLR4 is a type-1 transmembrane protein containing 799 amino acid residues. The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of IL-1 receptor. TLR4 is a receptor for lipopolysaccharide (LPS). In mice that are genetically tolerant to endotoxin, mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB.
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody
PTM N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

Images (1) Click the Picture to Zoom In

  • ARG20469 anti-CD284 / TLR4 antibody WB image

    Western blot: Rat kidney tissue lysate stained with ARG20469 anti-CD284 / TLR4 antibody.