ARG20515

anti-CD284 / TLR4 antibody

anti-CD284 / TLR4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CD284 / TLR4
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name CD284 / TLR4
Antigen Species Human
Immunogen Synthetic peptide around aa. 420-435 of Human TLR4.
Conjugation Un-conjugated
Full Name toll-like receptor 4
Alternate Names CD284; CD antigen CD284; ARMD10; hToll; TLR-4; TOLL; Toll-like receptor 4

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:50
IHC-P1:50
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 96 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 0.05% BSA
Preservative 0.05% Sodium azide
Stabilizer 0.05% BSA
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21898 Mouse TLR4

GeneID: 7099 Human TLR4

Swiss-port # O00206 Human Toll-like receptor 4

Swiss-port # Q9QUK6 Mouse Toll-like receptor 4

Gene Symbol TLR4
Gene Full Name toll-like receptor 4
Background The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediatiors of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).

1. JMuzio M., Natoli G. , Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101.
2. Medzhitov R. and Janeway C.A (1997). Cell 91: 295-298.
3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta 1518 (1-2): 157-161).
4. Takeuchi O., et al. (1999) Immunity 11: 443.
5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA 97: 2163-2167.
6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature 388 (6640): 394-397.
Function Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+require non-conserved histidines and are, therefore, species-specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody
PTM N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

Images (5) Click the Picture to Zoom In

  • ARG20515 anti-CD284 / TLR4 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse spleen tissue stained with ARG20515 anti-CD284 / TLR4 antibody.

  • ARG20515 anti-CD284 / TLR4 antibody WB image

    Western blot: TLR4 protein stained with ARG20515 anti-CD284 / TLR4 antibody at 1:1000 dilution.

  • ARG20515 anti-CD284 / TLR4 antibody IHC image

    Immunohistochemistry: Formalin-fixed Mouse colon colitis stained with ARG20515 anti-CD284 / TLR4 antibody at 1:100,000 for 12 hours at 4°C. Secondary antibody: Biotin Goat anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200 µl for 2 min at RT.

  • ARG20515 anti-CD284 / TLR4 antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG20515 anti-CD284 / TLR4 antibody at 1:100 dilution.

  • ARG20515 anti-CD284 / TLR4 antibody WB image

    Western blot: TLR4 protein stained with ARG20515 anti-CD284 / TLR4 antibody at 1:1000 dilution.