ARG62798

anti-CD29 / Integrin beta 1 antibody [MEM-101A]

anti-CD29 / Integrin beta 1 antibody [MEM-101A] for Flow cytometry,Immunoprecipitation,Western blot and Human,Pig,Dog

Developmental Biology antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-101A] recognizes CD29 / Integrin beta 1
Tested Reactivity Hu, Dog, Pig
Species Does Not React With Ms
Tested Application FACS, IP, WB
Specificity The clone MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).
HLDA VI; WS Code AS A048
Host Mouse
Clonality Monoclonal
Clone MEM-101A
Isotype IgG1
Target Name CD29 / Integrin beta 1
Antigen Species Human
Immunogen Raji Burkitt's lymphoma cell line
Conjugation Un-conjugated
Alternate Names CD29; Glycoprotein IIa; Fibronectin receptor subunit beta; VLAB; MSK12; CD antigen CD29; FNRB; GPIIA; VLA-4 subunit beta; VLA-BETA; MDF2; Integrin beta-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 µg/ml
IPAssay-dependent
WB2 µg/ml
Application Note WB: Sample preparation: Buffer with laurylmaltoside, 2x non-reducing SDS Application note: Non-reducing condition. SDS-PAGE (6% separating gel, 4% stacking gel). Recommended secondary antibody - anti mouse IgG/HRP, dilution 1:2000, 60 min on vertical incubator.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: Jurkat and Kg-1a

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3688 Human ITGB1

GeneID: 397019 Pig ITGB1

Swiss-port # P05556 Human Integrin beta-1

Swiss-port # Q9GLP0 Pig Integrin beta-1

Gene Symbol ITGB1
Gene Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Background CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.
Function Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion.
Isoform 5: Isoform 5 displaces isoform 1 in striated muscles. [UniProt]
Research Area Developmental Biology antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 88 kDa
PTM The cysteine residues are involved in intrachain disulfide bonds.

Images (1) Click the Picture to Zoom In

  • ARG62798 anti-CD29 / Integrin beta 1 antibody [MEM-101A] WB image

    Western blot: 1. Human PBL (peripheral blood lymphocytes) lysate, 2. Canine PBL lysate, 3. Porcine PBL lysate stained with ARG62798 anti-CD29 / Integrin beta 1 antibody [MEM-101A].

Clone References

Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs.

FACS / Dog

Alves EG et al.
BMC Vet Res.,  (2014)

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Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs.

Linard C et al.
Stem Cells Transl Med.,  (2013)

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hr_line

CD36 recruits α₅β₁ integrin to promote cytoadherence of P. falciparum-infected erythrocytes.

Davis SP et al.
PLoS Pathog.,  (2013)

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hr_line

Recombinant viral protein VP1 suppresses HER-2 expression and migration/metastasis of breast cancer.

Hung SW et al.
Breast Cancer Res Treat.,  (2012)

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hr_line

Creation of an in vitro microenvironment to enhance human fetal synovium-derived stem cell chondrogenesis.

FACS / Human

Li J et al.
Cell Tissue Res.,  (2011)

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