ARG67246
anti-CD3 antibody [SQab30364]
anti-CD3 antibody [SQab30364] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Monoclonal antibody [SQab30364] recognizes CD3. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab30364 |
| Isotype | IgG |
| Target Name | CD3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to Human CD3E(a.a. 1-100). |
| Conjugation | Un-conjugated |
| Alternate Names | CD3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3-Epsilon; CD3epsilon; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3; CD3e Molecule; CD3e Antigen; IMD18; TCRE |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain |
|---|---|
| Gene Symbol | CD3E |
| Gene Full Name | CD3 Epsilon Subunit Of T-Cell Receptor Complex |
| Background | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008] |
| Function | In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx. [Uniprot] |
| Cellular Localization | Cell membrane, Membrane. [Uniprot] |
| Calculated MW | 21 kDa |
| PTM | Disulfide bond, Phosphoprotein. [Uniprot] |
Images (2) Click the Picture to Zoom In
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ARG67246 anti-CD3 antibody [SQab30364] IHC-P image
Immunohistochemistry: Human Tonsil stained with ARG67246 anti-CD3 antibody [SQab30364] at 1:100 dilution.
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ARG67246 anti-CD3 antibody [SQab30364] IHC-P image
Immunohistochemistry: Human Appendix stained with ARG67246 anti-CD3 antibody [SQab30364] at 1:100 dilution.
