ARG52748

anti-CD31 antibody

anti-CD31 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Human,Mouse,Pig,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes CD31

Tested Reactivity Hu, Ms, Rat, Pig
Tested Application ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD31
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminus of mouse CD31 protein.
Conjugation Un-conjugated
Alternate Names EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:25 - 1:100
IHC-FoFr 1:25 - 1:50
IHC-Fr1:50
IHC-P1:50
WB1:100 -1:1000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Tonsil
Observed Size ~ 120 kDa

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18613 Mouse PECAM1

GeneID: 29583 Rat PECAM1

GeneID: 396941 Pig PECAM1

Background CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Function CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis.

Isoform Delta15: Does not protect against apoptosis. [UniProt]
Cellular Localization Membrane
Highlight Related Antibody Duos and Panels:
ARG30300 Endothelial Cell Marker Antibody Duo (CD31, CD34)
ARG30327 Microvascular Density Antibody Panel
Related products:
CD31 antibodies; CD31 ELISA Kits; CD31 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
Calculated MW 83 kDa
PTM Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.

Images (5) Click the Picture to Zoom In

  • ARG52748 anti-CD31 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and Paraffin-embedded MDA-MB-231 (tumor xenograft study) stained with ARG52748 anti-CD31 antibody.
    Antigen Retrieval Buffer: Bond Epitope Retrieval Solution.

  • ARG52748 anti-CD31 antibody WB image

    Western blot: 30 µg of HUVEC lysate stained with ARG52748 anti-CD31 antibody at 1:500 dilution.

  • ARG52748 anti-CD31 antibody IHC-P image

    WB analysis of LLC xenograft mouse tumor tissue lysate stained with ARG52748 anti-CD31 antibody.

    Reproduced under the Creative Commons license from Xu Fei et al. J Exp Clin Cancer Res., (2018) 37:207 Doi: 10.1186/s13046-018-0878-0, Fig. 7 A-B.

  • ARG52748 anti-CD31 antibody WB image

    WB analysis of LLC xenograft mouse tumor tissue lysate stained with ARG52748 anti-CD31 antibody.

    Reproduced under the Creative Commons license from Xu Fei et al. J Exp Clin Cancer Res., (2018) 37:207 Doi: 10.1186/s13046-018-0878-0, Fig. 7C.

  • ARG52748 anti-CD31 antibody IHC image

    Cryostat sections from Mouse Retinal stained with ARG52748 anti-CD31 antibody.

    Reproduced under the Creative Commons license from Lin Chin-I et al. July/August 2019, 6(4) ENEURO.0391-18.2019 1–17, Doi: 10.1523/ENEURO.0391-18.2019, Fig. 4 A-C.

Customer's Feedback

nuts_pic      Good

Review for anti-CD31 antibody

Application:WB

Sample:HUVEC

Sample Loading Amount:30 µl

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-CD31 antibody

Application:IHC

Sample:MDA-MB-231(tumor xenograft study)

Sample Preparation Method:Paraffin-embedded tissue

Fixation Buffer:Formalin

Fixation Time:-

Fixation Temperature:- ºC

Antigen Retrieval Buffer:Bond Epitope Retrieval Solution

Antigen Retrieval Time:20 min

Antigen Retrieval Temperature:100 ºC

Permeabilization Buffer:Peroxide

Primary Antibody Dilution Factor:1:50

Primary Antibody Incubation Time:30 min

Primary Antibody Incubation Temperature:25 ºC

Specific References

VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

IHC-P / Mouse

Qiu-Yue Lin et al.
Clin Transl Med.,  (2021)

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G6PD-NF-κB-HGF Signal in Gastric Cancer-Associated Mesenchymal Stem Cells Promotes the Proliferation and Metastasis of Gastric Cancer Cells by Upregulating the Expression of HK2.

IHC-P / Mouse

Bin Chen et al.
Frontiers in Oncology.,  (2021)

publication_link

 

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Exploring the mechanism of Bushenhuoxue formula acting on postmenopausal osteoporosis via network pharmacology and experimental validation.

IHC-P / Mouse

Chunhai Ke et al.
Preprint.,  (2021)

publication_link

 

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Therapeutic targeting of circ-CUX1/EWSR1/MAZ axis inhibits glycolysis and neuroblastoma progression.

IHC-P / Human

Huanhuan Li et al.
EMBO Mol Med.,  (2019)

publication_link

 

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NIR-cleavable drug adducts of gold nanostars for overcoming multidrug-resistant tumors.

IHC-Fr / Mouse

Andrea C Del Valle et al.
Biomater Sci.,  (2020)

publication_link

 

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Amygdalin Promotes Fracture Healing through TGF- β/Smad Signaling in Mesenchymal Stem Cells.

IHC-P / Mouse

Jun Ying et al.
Stem Cells Int.,  (2020)

publication_link

 

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Platelet-rich plasma promotes bone formation, restrains adipogenesis and accelerates vascularization to relieve steroids-induced osteonecrosis of the femoral head.

IHC-P / Rat

Hui-Hui Xu et al.
Platelets.,  (2020)

publication_link

 

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Bushenhuoxue formula accelerates fracture healing via upregulation of TGF-β/Smad2 signaling in mesenchymal progenitor cells.

IHC-P / Mouse

Hu Songfeng et al.
Phytomedicine.,  (2020)

publication_link

 

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Peripheral Blood Stem Cell Therapy Does Not Improve Outcomes of Femoral Head Osteonecrosis With Cap-Shaped Separated Cartilage Defect.

IHC-P / Human

Ying Jun et al.
J Orthop Res.,  (2019)

publication_link

 

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N-acetyl cysteine-loaded graphene oxide-collagen hybrid membrane for scarless wound healing.

IHC-P / Rat

Li Jialun et al.
Theranostics.,  (2019)

publication_link

 

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Blue Light Promotes Neurite Outgrowth of Retinal Explants in Postnatal ChR2 Mice.

IHC-Fr / Mouse

Lin Chin-I et al.
eNeuro.,  (2019)

publication_link

 

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Polydopamine-coated gold nanostar for combined antitumor and antiangiogenic therapy in multidrug-resistant breast cancer.

IHC-Fr / Mouse

You You-Hong et al.
Nanotheranostics.,  (2019)

publication_link

 

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Enhancing Microcirculation on Multitriggering Manner Facilitates Angiogenesis and Collagen Deposition on Wound Healing by Photoreleased NO from Hemin-Derivatized Colloids.

IHC-P / Mouse

Su Chia-Hao et al.
ACS Nano.,  (2019)

publication_link

 

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Cis-acting circ-CTNNB1 promotes β-catenin signaling and cancer progression via DDX3-mediated transactivation of YY1.

IHC-P / Mouse

Yang Feng et al.
Cancer Res.,  (2018)

publication_link

 

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Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes.

IHC-P / Mouse

Chen Yajun et al.
Cell Death Differ.,  (2018)

publication_link

 

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Astragaloside IV inhibits lung cancer progression and metastasis by modulating macrophage polarization through AMPK signaling

IHC-P / Mouse

Xu Fei et al.
J Exp Clin Cancer Res.,  (2018)

publication_link

 

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Stiffness Modification of Photopolymerizable Gelatin-Methacrylate Hydrogels Influences Endothelial Differentiation of Human Mesenchymal Stem Cells.

ICC/IF / Human

Lin Chih-Hsin et al.
J Tissue Eng Regen Med.,  (2018)

publication_link

 

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Obesity enhances carcinogen 7, 12-Dimethylbenz [a] anthracene -induced tumorigenesis in vitro and in vivo.

IHC-P / Mouse

Hsieh Chia-Chien et al.
Food Chem Toxicol.,  (2017)

publication_link

 

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Administration of erythropoietin prevents bone loss in osteonecrosis of the femoral head in mice

IHC-P / Mouse

Xu Taotao et al.
Molecular Medicine Reports.,  (2017)

publication_link

 

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CO2 Delivery to Accelerate Incisional Wound Healing Following Single Irradiation of Near-Infrared Lamp on the Coordinated Colloids

IHC-P / Mouse

Wei-Peng Li et al.
ACS Nano. ,  (2017)

publication_link

 

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Multifunctional Hydrogels Prepared by Dual Ion Cross-Linking for Chronic Wound Healing.

IHC-P / Mouse

Li Y et al.
ACS Appl Mater Interfaces.,  (2017)

publication_link

 

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Injectable bioactive akermanite/alginate composite hydrogels for in situ skin tissue engineering

IHC-Fr / Mouse

Han Y et al.
J. Mater. Chem. B.,  (2017)

publication_link

 

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