anti-CD31 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Human,Mouse,Pig,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
Rabbit Polyclonal antibody recognizes CD31
|Tested Reactivity||Hu, Ms, Rat, Pig|
|Tested Application||ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB|
|Immunogen||Synthetic peptide corresponding to C-terminus of mouse CD31 protein.|
|Alternate Names||EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Observed Size||~ 120 kDa|
|Purification||Immunogen affinity purified|
|Buffer||PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide|
|Preservative||< 0.1% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]|
|Function||CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis.
Isoform Delta15: Does not protect against apoptosis. [UniProt]
|Highlight||Related Antibody Duos and Panels:
ARG30300 Endothelial Cell Marker Antibody Duo (CD31, CD34)
ARG30327 Microvascular Density Antibody Panel
CD31 antibodies; CD31 ELISA Kits; CD31 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody|
|Calculated MW||83 kDa|
|PTM||Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Images (5) Click the Picture to Zoom In
ARG52748 anti-CD31 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and Paraffin-embedded MDA-MB-231 (tumor xenograft study) stained with ARG52748 anti-CD31 antibody.
Antigen Retrieval Buffer: Bond Epitope Retrieval Solution.
ARG52748 anti-CD31 antibody WB image
Western blot: 30 µg of HUVEC lysate stained with ARG52748 anti-CD31 antibody at 1:500 dilution.
Review for anti-CD31 antibody
Sample：MDA-MB-231(tumor xenograft study)
Sample Preparation Method：Paraffin-embedded tissue
Fixation Temperature：- ºC
Antigen Retrieval Buffer：Bond Epitope Retrieval Solution
Antigen Retrieval Time：20 min
Antigen Retrieval Temperature：100 ºC
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：30 min
Primary Antibody Incubation Temperature：25 ºC