ARG52756

anti-CD34 antibody [QBEND/10]

anti-CD34 antibody [QBEND/10] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Primates,Rabbit

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
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Overview

Product Description

Mouse Monoclonal antibody [QBEND/10] recognizes CD34 (Endothelial Cell Marker)

Tested Reactivity Hu, Ms, NHuPrm, Rb
Species Does Not React With Rat, Bov, Dog, Sheep
Tested Application FACS, IHC-Fr, IHC-P, IP, WB
Specificity The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND/10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. The clone QBEND/10 recognises the Class II epitope on CD34.
Host Mouse
Clonality Monoclonal
Clone QBEND/10
Isotype IgG1, kappa
Target Name CD34
Antigen Species Human
Immunogen Detergent solubilized vesicular suspension prepared from a perfusate of human term placenta.
Conjugation Un-conjugated
Alternate Names Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
IHC-FrAssay-Dependent
IHC-P1:200
IPAssay-Dependent
WBAssay-dependent
Application Note IHC-P:
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Lung, Tonsil
Observed Size 105-120 kDa

Properties

Form Liquid
Buffer Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12490 Mouse CD34

GeneID: 947 Human CD34

Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34

Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34

Gene Symbol CD34
Gene Full Name CD34 molecule
Background CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
Cellular Localization Cytoplasm, Membrane
Highlight Related Antibody Duos and Panels:
ARG30300 Endothelial Cell Marker Antibody Duo (CD31, CD34)
ARG30328 Angiogenesis Antibody Panel
Related products:
CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Vascular development is regulated by FGF-dependent metabolic control
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Calculated MW 41 kDa
PTM Highly glycosylated.
Phosphorylated on serine residues by PKC.

Images (6) Click the Picture to Zoom In

  • ARG52756 anti-CD34 antibody [QBEND/10] WB image

    Western blot: 30 µg of mouse lung lysate stained with ARG52756 anti-CD34 antibody [QBEND/10] at 1:500 dilution.

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Breast Ductal Carcinoma stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Cervical Squamous Cell carcinoma stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Colon stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Placenta stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Tonsil stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

Customer's Feedback

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Review for anti-CD34 antibody [QBEND/10]

Application:WB

Sample:Mouse lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Role of Angiogenesis in Chronic Radiation Proctitis: New Evidence Favoring Inhibition of Angiogenesis Ex Vivo.

IHC-P / Human

Wu Pei-Huang et al.
Dig Dis Sci.,  (2017)

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