ARG66322

anti-CD34 antibody [SQab1870]

anti-CD34 antibody [SQab1870] for IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1870] recognizes CD34
Tested Reactivity Hu
Tested Application FACS, IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab1870
Isotype IgG
Target Name CD34
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of CD34.
Conjugation Un-conjugated
Full Name CD34 molecule
Alternate Names Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:200
IHC-P1:800 - 1:1600
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 41 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 947 Human CD34

Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34

Gene Symbol CD34
Gene Full Name CD34 molecule
Background CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
Highlight Related products:
CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
PTM Highly glycosylated.

Phosphorylated on serine residues by PKC. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66322 anti-CD34 antibody [SQab1870] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 mins. The cells were stained with ARG66322 anti-CD34 antibody [SQab1870] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66322 anti-CD34 antibody [SQab1870] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta tissue stained with ARG66322 anti-CD34 antibody [SQab1870] at 1:1,600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.