anti-CD34 antibody [SQab1870]
anti-CD34 antibody [SQab1870] for IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1870] recognizes CD34|
|Tested Application||FACS, IHC-P|
|Immunogen||Synthetic peptide around the C-terminus of CD34.|
|Alternate Names||Hematopoietic progenitor cell antigen CD34; CD antigen CD34|
|Application Note||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||CD34 molecule|
|Background||CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]|
|Function||CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]|
CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody|
|Calculated MW||41 kDa|
Phosphorylated on serine residues by PKC. [UniProt]
Images (2) Click the Picture to Zoom In
ARG66322 anti-CD34 antibody [SQab1870] FACS image
Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 mins. The cells were stained with ARG66322 anti-CD34 antibody [SQab1870] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
ARG66322 anti-CD34 antibody [SQab1870] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta tissue stained with ARG66322 anti-CD34 antibody [SQab1870] at 1:1,600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.