anti-CD35 / CR1 antibody [E11] (PE)

anti-CD35 / CR1 antibody [E11] (PE) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot,ELISA and Human,Rhesus Monkey,Chimpanzee,Baboon

Developmental Biology antibody; Immune System antibody


Product Description PE-conjugated Mouse Monoclonal antibody [E11] recognizes CD35 / CR1
Tested Reactivity Hu, Bb, Chimp, R. Mk
Tested Application ELISA, FACS, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone E11
Isotype IgG1, kappa
Target Name CD35 / CR1
Antigen Species Human
Immunogen Acute monocytic leukemia cells and normal blood monocytes
Conjugation PE
Alternate Names C3b/C4b receptor; C4BR; CD antigen CD35; KN; CD35; C3BR; Complement receptor type 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl/10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Buffer PBS, 0.1% Sodium azide and Sucrose.
Preservative 0.1% Sodium azide
Stabilizer Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1378 Human CR1

Swiss-port # P17927 Human Complement receptor type 1

Gene Symbol CR1
Gene Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Background CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.
Function Mediates cellular binding of particles and immune complexes that have activated complement. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 224 kDa

Clone References

Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor.


Park HJ et al.
J Biol Chem.,  (2014)




Effect of B-cell depletion on coreceptor switching in R5 simian-human immunodeficiency virus infection of rhesus macaques.

IHC / Human

Tasca S et al.
J Virol.,  (2011)




Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum.

ELISA / Human

LaPan P et al.
BMC Pulm Med.,  (2010)




Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).

Hogg N et al.
Eur J Immunol.,  (1984)




Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML.

Linch DC et al.
Blood.,  (1984)