ARG20926

anti-CD40 antibody [1C10] (Biotin)

anti-CD40 antibody [1C10] (Biotin) for Flow cytometry,Cellular activation ,Blocking and Mouse

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody

Overview

Product Description Biotin-conjugated Rat Monoclonal antibody [1C10] recognizes CD40
Tested Reactivity Ms
Tested Application BL, Cell-Act , FACS
Specificity Mouse CD40.
Host Rat
Clonality Monoclonal
Clone 1C10
Isotype IgG2a, kappa
Target Name CD40
Species Mouse
Immunogen sCD40
Conjugation Biotin
Full Name CD40 antigen
Alternate Names CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
Cell-Act Assay-dependent
FACS< 2 µg/10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 31 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21939 Mouse CD40

Swiss-port # P27512 Mouse Tumor necrosis factor receptor superfamily member 5

Gene Symbol CD40
Gene Full Name CD40 antigen
Background This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
Function Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody