ARG21361

anti-CD43 antibody [DF-T1] (PE)

anti-CD43 antibody [DF-T1] (PE) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot and Human

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [DF-T1] recognizes CD43
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Human CD43.
Host Mouse
Clonality Monoclonal
Clone DF-T1
Isotype IgG1, kappa
Target Name CD43
Antigen Species Human
Immunogen KG-1 cells
Conjugation PE
Full Name sialophorin
Alternate Names LSN; CD43; GALGP; GPL115

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 40 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6693 Human SPN

Swiss-port # P16150 Human Leukosialin

Gene Symbol SPN
Gene Full Name sialophorin
Background The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]
Function One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21361 anti-CD43 antibody [DF-T1] (PE) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG21361 anti-CD43 antibody [DF-T1] (PE) and ARG21258 anti-CD19 antibody [SJ25-C1] (FITC).