anti-CD45 antibody [I3/2.3]

anti-CD45 antibody [I3/2.3] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Mouse

Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
publication_link Publication2


Product Description

Rat Monoclonal antibody [I3/2.3] recognizes CD45

Tested Reactivity Ms
Tested Application FACS, IHC-Fr, IP
Host Rat
Clonality Monoclonal
Clone I3/2.3
Isotype IgG2b, lambda
Target Name CD45
Antigen Species Mouse
Immunogen T1M1 (Thy-1─c) cells
Conjugation Un-conjugated
Alternate Names LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC; B220

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1:50 - 1:300
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 19264 Mouse PTPRC

Gene Symbol Ptprc
Gene Full Name protein tyrosine phosphatase, receptor type, C
Background CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Function CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
Calculated MW 147 kDa
PTM Heavily N- and O-glycosylated.

Images (1) Click the Picture to Zoom In

  • ARG20565 anti-CD45 antibody [I3/2.3] FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20565 anti-CD45 antibody [I3/2.3] followed by ARG21709 Mouse anti-Rat IgG2b antibody [2B10A8] (PE).

Specific References

The selection of photoinitiators for photopolymerization of biodegradable polymers and its application in digital light processing additive manufacturing

IHC-Fr / Mouse

Chia-Chun Wang et al.
J Biomed Mater Res A.,  (2021)




The Effect of Heat Treatment toward Glycerol-Based, Photocurable Polymeric Scaffold: Mechanical, Degradation and Biocompatibility.

IHC-Fr / Mouse

Wai-Sam Ao-Ieong et al.
Polymers (Basel).,  (2021)