anti-CD45 antibody [SQab1881]
anti-CD45 antibody [SQab1881] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1881] recognizes CD45|
|Tested Application||FACS, IHC-P, WB|
|Immunogen||Synthetic peptide corresponding to aa. 300-400 (intracellular) of Human CD45.|
|Full Name||protein tyrosine phosphatase, receptor type, C|
|Alternate Names||LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 188.8.131.52; B220|
|Application Note||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Calculated MW||147 kDa|
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||protein tyrosine phosphatase, receptor type, C|
|Background||The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]|
|Function||Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). [UniProt]|
CD45 antibodies; CD45 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
|PTM||Heavily N- and O-glycosylated. [UniProt]|
Images (3) Click the Picture to Zoom In
ARG66333 anti-CD45 antibody [SQab1881] FACS image
Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66333 anti-CD45 antibody [SQab1881] (red) at 1:2,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
ARG66333 anti-CD45 antibody [SQab1881] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG66333 anti-CD45 antibody [SQab1881] at 1:800 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
ARG66333 anti-CD45 antibody [SQab1881] WB image
Western blot: 10 µg of Jurkat and Raji cell lysates stained with ARG66333 anti-CD45 antibody [SQab1881] at 1:1000 dilution.