anti-CD45RA antibody [MEM-56] (PerCP)

anti-CD45RA antibody [MEM-56] (PerCP) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody


Product Description

PerCP-conjugated Mouse Monoclonal antibody [MEM-56] recognizes CD45RA

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-56 reacts with CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.
HLDA IV; WS Code NL 907
Host Mouse
Clonality Monoclonal
Clone MEM-56
Isotype IgG2b
Target Name CD45RA
Antigen Species Human
Immunogen Human thymocytes and T lymphocytes.
Conjugation PerCP
Alternate Names LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC; B220

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5788 Human PTPRC

Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C

Gene Symbol PTPRC
Gene Full Name protein tyrosine phosphatase, receptor type, C
Background CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Function CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
Calculated MW 147 kDa
PTM Heavily N- and O-glycosylated.

Images (1) Click the Picture to Zoom In

  • ARG54279 anti-CD45RA antibody [MEM-56] (PerCP) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG54279 anti-CD45RA antibody [MEM-56] (PerCP).

Clone References

Origins of tumor-associated macrophages and neutrophils.

FACS / Human

Cortez-Retamozo V et al.
Proc Natl Acad Sci U S A.,  (2012)




Reversible senescence in human CD4+CD45RA+CD27- memory T cells.

FACS / Human

Di Mitri D et al.
J Immunol.,  (2011)




High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo.

FACS / Human

Simmons R et al.
J Infect Dis.,  (2011)




Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma.

ICC/IF / Human

Abdulamir AS et al.
BMC Immunol.,  (2008)