anti-CD46 antibody [MEM-258] (APC)

anti-CD46 antibody [MEM-258] (APC) for Flow cytometry and Human,Bovine

Immune System antibody


Product Description APC-conjugated Mouse Monoclonal antibody [MEM-258] recognizes CD46
Tested Reactivity Hu, Bov
Tested Application FACS
Specificity The clone MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast; it is negative on erythrocytes.
Host Mouse
Clonality Monoclonal
Clone MEM-258
Isotype IgG1
Target Name CD46
Antigen Species Human
Immunogen HPB-ALL human T cell line
Conjugation APC
Alternate Names MIC10; TLX; CD antigen CD46; Trophoblast leukocyte common antigen; AHUS2; TRA2.10; Membrane cofactor protein; MCP

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 280851 Bovine CD46

GeneID: 4179 Human CD46

Swiss-port # P15529 Human Membrane cofactor protein

Swiss-port # Q6VE48 Bovine Membrane cofactor protein

Gene Symbol CD46
Gene Full Name CD46 molecule, complement regulatory protein
Background CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
Function Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. [UniProt]
Research Area Immune System antibody
Calculated MW 44 kDa
PTM N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK.

Images (1) Click the Picture to Zoom In

  • ARG53863 anti-CD46 antibody [MEM-258] (APC) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53863 anti-CD46 antibody [MEM-258] (APC).

Clone References

Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection.

WB / Human

Lee MS et al.
PLoS Pathog.,  (2014)




Use of SLAM and PVRL4 and identification of pro-HB-EGF as cell entry receptors for wild type phocine distemper virus.


Melia MM et al.
PLoS One.,  (2014)




Antimicrobial host defence peptide, LL-37, as a potential vaginal contraceptive.

FACS / Human

Srakaew N et al.
Hum Reprod.,  (2014)




Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration.

FACS / Human

Kolev MV et al.
Pharmacogenomics J.,  (2010)




Transduction of liver metastases after intravenous injection of Ad5/35 or Ad35 vectors with and without factor X-binding protein pretreatment.

ICC/IF / Mouse

Liu Y et al.
Hum Gene Ther.,  (2009)




Tumor and vascular targeting of a novel oncolytic measles virus retargeted against the urokinase receptor.


Jing Y et al.
Cancer Res.,  (2009)