ARG21028

anti-CD49b / Integrin alpha 2 antibody [DX5] (Biotin)

anti-CD49b / Integrin alpha 2 antibody [DX5] (Biotin) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Mouse

Overview

Product Description Biotin-conjugated Rat Monoclonal antibody [DX5] recognizes CD49b / Integrin alpha 2
Tested Reactivity Ms
Tested Application FACS, IHC-Fr, IHC-P
Specificity The clone DX5 reacts with CD49b, also known as very late antigen-2 (VLA-2) and α2 integrin. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). The clone DX5 has not been demonstrated to have activating or blocking activity.
Host Rat
Clonality Monoclonal
Clone DX5
Isotype IgM, kappa
Target Name CD49b / Integrin alpha 2
Antigen Species Mouse
Immunogen NK cells isolated from C57BL/6 mice
Conjugation Biotin
Full Name integrin alpha 2
Alternate Names Collagen receptor; VLA-2 subunit alpha; HPA-5; CD49B; CD49 antigen-like family member B; GPIa; VLA-2; CD antigen CD49b; BR; VLAA2; Platelet membrane glycoprotein Ia; Integrin alpha-2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 129 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16398 Mouse ITGA2

Swiss-port # Q62469 Mouse Integrin alpha-2

Gene Symbol ITGA2
Gene Full Name integrin alpha 2
Background This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Function Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21028 anti-CD49b / Integrin alpha 2 antibody [DX5] (Biotin) FACS image

    Flow Cytometry: C57BL/6 Mouse splenocytes stained with ARG21028 anti-CD49b / Integrin alpha 2 antibody [DX5] (Biotin) and ARG65527 anti-CD161bc / NK1.1 antibody [PK136] (PE) followed by Streptavidin (FITC).