anti-CD49d / LPAM1 / Integrin alpha 4 antibody [DATK32]

anti-CD49d / LPAM1 / Integrin alpha 4 antibody [DATK32] for Blocking,Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Mouse


Product Description Rat Monoclonal antibody [DATK32] recognizes CD49d / LPAM1 / Integrin alpha 4
Tested Reactivity Ms
Tested Application BL, FACS, IHC-Fr, IP
Specificity Mouse LPAM-1. The clone DATK32 recognizes a conformational epitope on the heterodimer.
Host Rat
Clonality Monoclonal
Clone DATK32
Isotype IgG2a, kappa
Target Name CD49d / LPAM1 / Integrin alpha 4
Antigen Species Mouse
Immunogen AKR/Cum mouse T lymphoma cell line TK-1
Conjugation Un-conjugated
Alternate Names CD49 antigen-like family member D; VLA-4 subunit alpha; CD49D; Integrin alpha-IV; CD antigen CD49d; Integrin alpha-4; IA4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 16401 Mouse ITGA4

Swiss-port # Q00651 Mouse Integrin alpha-4

Gene Symbol ITGA4
Gene Full Name integrin alpha 4
Background The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008]
Function Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. [UniProt]
Calculated MW 115 kDa
PTM Phosphorylation on Ser-1027 inhibits PXN binding.

Images (1) Click the Picture to Zoom In

  • ARG21042 anti-CD49d / LPAM1 / Integrin alpha 4 antibody [DATK32] FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21042 anti-CD49d / LPAM1 / Integrin alpha 4 antibody [DATK32] followed by ARG23825 Mouse anti-Rat IgG2a antibody [2A8F4] (Cy5).