anti-CD5 antibody [CRIS1] (PE)
anti-CD5 antibody [CRIS1] (PE) for Flow cytometry and Human
Developmental Biology antibody; Immune System antibody
|Product Description||PE-conjugated Mouse Monoclonal antibody [CRIS1] recognizes CD5|
|Specificity||The clone CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
HLDA I; WS Code T 29
HLDA III; WS Code T 530
|Immunogen||stimulated human leukocytes|
|Alternate Names||CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification Note||The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.|
|Buffer||PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA|
|Preservative||15 mM Sodium azide|
|Stabilizer||0.2% (w/v) high-grade protease free BSA|
|Storage Instruction||Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||CD5 molecule|
|Background||CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
|Function||May act as a receptor in regulating T-cell proliferation. [UniProt]|
|Research Area||Developmental Biology antibody; Immune System antibody|
|Calculated MW||55 kDa|
|PTM||Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.|
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