anti-CD5 antibody [CRIS1] (PE)

anti-CD5 antibody [CRIS1] (PE) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody


Product Description PE-conjugated Mouse Monoclonal antibody [CRIS1] recognizes CD5
Tested Reactivity Hu
Tested Application FACS
Specificity The clone CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
HLDA I; WS Code T 29
HLDA III; WS Code T 530
Host Mouse
Clonality Monoclonal
Clone CRIS1
Isotype IgG2a
Target Name CD5
Antigen Species Human
Immunogen stimulated human leukocytes
Conjugation PE
Alternate Names CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 921 Human CD5

Swiss-port # P06127 Human T-cell surface glycoprotein CD5

Gene Symbol CD5
Gene Full Name CD5 molecule
Background CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Function May act as a receptor in regulating T-cell proliferation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 55 kDa
PTM Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.

Images (1) Click the Picture to Zoom In

  • ARG53868 anti-CD5 antibody [CRIS1] (PE) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG53868 anti-CD5 antibody [CRIS1] (PE).

Clone References

Conformation of the hypervariable region L3 without the key proline residue.

Guarné A et al.
Protein Sci.,  (1996)




The clusters of differentiation (CD) defined by the First International Workshop on Human Leucocyte Differentiation Antigens.

Bernard A et al.
Hum Immunol.,  (1984)




Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis.

Arrizabalaga P et al.
Nephron.,  (1989)




Intracellular events involved in CD5-induced human T cell activation and proliferation.

Alberola-Ila J et al.
J Immunol.,  (1992)