anti-CD5 antibody [SQab20184]
anti-CD5 antibody [SQab20184] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab20184] recognizes CD5|
|Tested Application||IHC-P, WB|
|Immunogen||Recombinant full length protein of Human CD5 (extracellular). The immunogen contains the specific extracellular domain (D32-P372).|
|Alternate Names||CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1|
|Application Note||IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||WB: Jurkat
IHC-P: Appendix tissue
|Observed Size||~ 52 kDa|
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||CD5 molecule|
|Background||This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]|
|Function||May act as a receptor in regulating T-cell proliferation. [UniProt]|
|Cellular Localization||Cell membrane; Single-pass type I membrane protein. [UniProt]|
|Calculated MW||55 kDa|
|PTM||Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1. [UniProt]|
Images (2) Click the Picture to Zoom In
ARG66751 anti-CD5 antibody [SQab20184] WB image (Customer's Feedback)
Western blot: 10 µg of Jurkat cell lysate stained with ARG66751 anti-CD5 antibody [SQab20184] at 1:1000 dilution.
ARG66751 anti-CD5 antibody [SQab20184] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human appendix tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66751 anti-CD5 antibody [SQab20184] at 18°C - 25°C for 30 minutes.