anti-CD50 / ICAM3 antibody [MEM-171] (PE)

anti-CD50 / ICAM3 antibody [MEM-171] (PE) for Flow cytometry and Human

Immune System antibody; Signaling Transduction antibody


Product Description PE-conjugated Mouse Monoclonal antibody [MEM-171] recognizes CD50 / ICAM3
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-171 recognizes an epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes.
HLDA VI; WS Code BP 614
HLDA VI; WS Code NL N-L022
Host Mouse
Clonality Monoclonal
Clone MEM-171
Isotype IgG1
Target Name CD50 / ICAM3
Antigen Species Human
Immunogen Human granulocytes
Conjugation PE
Alternate Names CD50; CD antigen CD50; Intercellular adhesion molecule 3; ICAM-3; ICAM-R; CDw50; CDW50

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3385 Human ICAM3

Swiss-port # P32942 Human Intercellular adhesion molecule 3

Gene Symbol ICAM3
Gene Full Name intercellular adhesion molecule 3
Background CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. [UniProt]
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 60 kDa
PTM Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.

Images (1) Click the Picture to Zoom In

  • ARG53869 anti-CD50 / ICAM3 antibody [MEM-171] (PE) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG53869 anti-CD50 / ICAM3 antibody [MEM-171] (PE).

Clone References

Lymphocytes can self-steer passively with wind vane uropods.

Valignat MP et al.
Nat Commun.,  (2014)




Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO).

Linnebacher M et al.
J Biomed Biotechnol.,  (2010)