ARG21381

anti-CD51 / Integrin alpha V antibody [13C2]

anti-CD51 / Integrin alpha V antibody [13C2] for Blocking,Depletion,ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Purification and Human,African green monkey,Bovine,Rabbit

Overview

Product Description Mouse Monoclonal antibody [13C2] recognizes CD51 / Integrin alpha V
Tested Reactivity Hu, AGMK, Bov, Rb
Tested Application BL, Depletion, ELISA, FACS, ICC/IF, IHC-Fr, IP, Puri
Specificity Human/African Green Monkey/Rabbit/Bovine CD51.
Host Mouse
Clonality Monoclonal
Clone 13C2
Isotype IgG1, kappa
Target Name CD51 / Integrin alpha V
Antigen Species Human
Immunogen Cell suspension containing osteoclasts from osteoclastomas
Conjugation Un-conjugated
Full Name integrin, alpha V
Alternate Names CD51; VNRA; CD antigen CD51; VTNR; Vitronectin receptor subunit alpha; Integrin alpha-V; MSK8

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
DepletionAssay-dependent
ELISAAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
PuriAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 116 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 281875 Bovine ITGAV

GeneID: 3685 Human ITGAV

Swiss-port # P06756 Human Integrin alpha-V

Swiss-port # P80746 Bovine Integrin alpha-V

Gene Symbol ITGAV
Gene Full Name integrin, alpha V
Background This gene encodes a protein that is a member of the integrin superfamily. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. This protein has been shown to heterodimerize with beta 1, beta 3, beta 5, beta 6, and beta 8; the heterodimer of alpha v and beta 3 is the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. It is proposed that this protein may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants that encode different protein isoforms. Note that the integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015]
Function The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21381 anti-CD51 / Integrin alpha V antibody [13C2] FACS image

    Flow Cytometry: Human HPV-16 E6/E7 transformed cell line HS-5 was stained with ARG21381 anti-CD51 / Integrin alpha V antibody [13C2] followed by ARG21565 Goat anti-Mouse IgG1 antibody (PE) (pre-adsorbed).