anti-CD53 antibody [MEM-53] (PE)

anti-CD53 antibody [MEM-53] (PE) for Flow cytometry and Human

Immune System antibody; Signaling Transduction antibody


Product Description

PE-conjugated Mouse Monoclonal antibody [MEM-53] recognizes CD53

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells.
MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
HLDA IV; WS Code NL 59
HLDA V; WS Code B CD53.5
HLDA V; WS Code BP BP287
HLDA V; WS Code T T-096
HLDA V; WS Code X XB004
Host Mouse
Clonality Monoclonal
Clone MEM-53
Isotype IgG1
Target Name CD53
Immunogen Leukocytes of pacient suffering from a LGL-type leukemia.
Conjugation PE
Alternate Names Tetraspanin-25; Leukocyte surface antigen CD53; Tspan-25; Cell surface glycoprotein CD53; CD antigen CD53; MOX44; TSPAN25

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 963 Human CD53

Swiss-port # P19397 Human Leukocyte surface antigen CD53

Gene Symbol CD53
Gene Full Name CD53 molecule
Background CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Function Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity). [UniProt]
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 24 kDa

Images (1) Click the Picture to Zoom In

  • ARG53872 anti-CD53 antibody [MEM-53] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53872 anti-CD53 antibody [MEM-53] (PE).

Clone References

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Drukker M et al.
Nat Biotechnol.,  (2012)




CD63 is not required for production of infectious human immunodeficiency virus type 1 in human macrophages.


Ruiz-Mateos E et al.
J Virol.,  (2008)




In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53.


Deneka M et al.
J Cell Biol.,  (2007)




CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells.

Olweus J et al.
J Immunol.,  (1993)




Cross-linking of CD53 promotes activation of resting human B lymphocytes.

Rasmussen AM et al.
J Immunol.,  (1994)