ARG21290

anti-CD54 / ICAM1 antibody [15.2]

anti-CD54 / ICAM1 antibody [15.2] for Blocking,ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Neutralizing,Western blot and Human,Pig

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody

Overview

Product Description Mouse Monoclonal antibody [15.2] recognizes CD54 / ICAM1
Tested Reactivity Hu, Pig
Tested Application BL, ELISA, FACS, ICC/IF, IHC-Fr, IP, Neut, WB
Specificity Human/Porcine CD54.
Host Mouse
Clonality Monoclonal
Clone 15.2
Isotype IgG1, kappa
Target Name CD54 / ICAM1
Antigen Species Human
Immunogen Rheumatoid synovial cells and human monocytes
Conjugation Un-conjugated
Full Name intercellular adhesion molecule 1
Alternate Names CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
NeutAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 58 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3383 Human ICAM1

Swiss-port # P05362 Human Intercellular adhesion molecule 1

Gene Symbol ICAM1
Gene Full Name intercellular adhesion molecule 1
Background This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
PTM Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.