ARG21384

anti-CD55 antibody [143-30]

anti-CD55 antibody [143-30] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [143-30] recognizes CD55
Tested Reactivity Hu
Tested Application FACS, IHC-Fr, IHC-P, IP, WB
Specificity Human CD55.
Host Mouse
Clonality Monoclonal
Clone 143-30
Isotype IgG1, kappa
Target Name CD55
Antigen Species Human
Immunogen PHA activated peripheral blood mononuclear cells
Conjugation Un-conjugated
Full Name CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Alternate Names DAF; CD antigen CD55; CROM; Complement decay-accelerating factor; CR; TC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 41 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1604 Human CD55

Swiss-port # P08174 Human Complement decay-accelerating factor

Gene Symbol CD55
Gene Full Name CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Background This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. [UniProt]
PTM The Ser/Thr-rich domain is heavily O-glycosylated.