ARG21391

anti-CD57 antibody [NK-1] (Biotin)

anti-CD57 antibody [NK-1] (Biotin) for Blocking,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Purification,Western blot and Human

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [NK-1] recognizes CD57
Tested Reactivity Hu
Tested Application BL, FACS, ICC/IF, IHC-Fr, IHC-P, Puri, WB
Specificity Human CD57.
Host Mouse
Clonality Monoclonal
Clone NK-1
Isotype IgM, kappa
Target Name CD57
Antigen Species Human
Immunogen Membrane extract of the human lymphoblastoid cell line HSB-2
Conjugation Biotin
Full Name beta-1,3-glucuronyltransferase 1
Alternate Names Glucuronosyltransferase P; CD57; LEU7; GlcAT-P; GLCATP; HNK1; GlcUAT-P; NK1; Beta-1,3-glucuronyltransferase 1; EC 2.4.1.135; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GLCUATP; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; NK-1

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
PuriAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 38 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 27087 Human B3GAT1

Swiss-port # Q9P2W7 Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

Gene Symbol B3GAT1
Gene Full Name beta-1,3-glucuronyltransferase 1
Background The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Function Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity). [UniProt]
PTM The soluble form derives from the membrane form by proteolytic processing.

Images (1) Click the Picture to Zoom In

  • ARG21391 anti-CD57 antibody [NK-1] (Biotin) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG21391 anti-CD57 antibody [NK-1] (Biotin) and Mouse anti-Human CD3 antibody (PE) followed by Streptavidin (FITC).