ARG20931

anti-CD61 / Integrin beta 3 antibody [PM6/13]

anti-CD61 / Integrin beta 3 antibody [PM6/13] for Blocking,ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [PM6/13] recognizes CD61 / Integrin beta 3
Tested Reactivity Hu
Tested Application BL, ELISA, FACS, ICC/IF, IP, WB
Specificity Human CD61.
Host Mouse
Clonality Monoclonal
Clone PM6/13
Isotype IgG1, kappa
Target Name CD61 / Integrin beta 3
Antigen Species Human
Immunogen Human platelet plasma membrane
Conjugation Un-conjugated
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Alternate Names GT; CD antigen CD61; CD61; BDPLT2; GPIIIa; BDPLT16; GP3A; Platelet membrane glycoprotein IIIa; Integrin beta-3

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 87 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3690 Human ITGB3

Swiss-port # P05106 Human Integrin beta-3

Gene Symbol ITGB3
Gene Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Background The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
Function Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt]
PTM Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.

Images (1) Click the Picture to Zoom In

  • ARG20931 anti-CD61 / Integrin beta 3 antibody [PM6/13] FACS image

    Flow Cytometry: Human peripheral blood platelets stained with ARG20931 anti-CD61 / Integrin beta 3 antibody [PM6/13] followed by ARG21565 Goat anti-Mouse IgG1 antibody (PE) (pre-adsorbed).